类先天性马蹄内翻足动物模型的建立及病理演变研究

Animal models of analogic congenital clubfoot and pathological research

目的 了解先天性马蹄内翻足的发病机制并探索其病理发生、发展规律而建立先天性马蹄内翻足的实验动物模型。方法 应用 6 3只雌雄性Wistar大白鼠从怀孕第 10d起将维甲酸 (12 0mg/kg)石蜡油悬液 (40mg/ml)经胃管单次注入 ,建立CCF胎鼠实验动物模型。 结果 实验动物模型的马蹄内翻足发病率为 5 3.7% ,单足为 79.4 % ,双足为 2 0 .6 %。胫骨远端、距骨持续停滞在胚胎阶段 ,距骨、跟骨间重叠不良和跟骨内翻 ,踝角与对照组相比随胎鼠发育而逐渐增大。结论 先天性马蹄内翻足在胚胎发育的重要时期即有足的马蹄内翻且畸形随生长发育而加重 ,其病因可能与脊髓神经病变相关。

Objective In order to explore etiology and the progress of development of congenital clubfoot. Methods Sixty three pregnant Wistar rats were administered with a suspension of Tretinoin (120?mg/kg)and liquid paraffin (40?mg/ml) on gestational days 10 and control group were studied. Results There were clubfoot like deformity formation in 53.7% of the experimental fetuses (unilateral 79.4% , bilateral 20.6% ). Stagnation of the embryonic stage of the talus and tibia in fetuses with severe clubfoot like deformity was observed. Faulty overlapping of talus and calcaneus, and the varus calcaneus were founded. The ankle angles were significantly larger than that in the control group. Conclusions The experimental results suggest that the rotational anomalies in the clubfoot are existed from the beginning of embryonic stage, and aggravated following the development. The etiology may be closely correlated with pathogenesis of spinal cord.