利用质粒指纹图谱法对大肠杆菌耐药质粒遗传特性的研究

Isolation and Characters of Antibiotic Plasmids from Chicken Escherichia coli

为研究鸡源大肠杆菌耐药性及耐药的生物学特性,选用30株来自不同地区的鸡源大肠杆菌,在药敏试验的基础上,对耐药菌质粒进行了指纹图谱分析。结果表明:30株鸡源大肠杆菌对氨苄青霉素、青霉素、四环素的耐药率为100 00%,对氯霉素、庆大霉素、利福平、红霉素的耐药率为60 00%～93 33%。多重耐药率为26 67%～80 00%。质粒图谱比较发现:来源相同的菌株质粒图谱相同或相似;来源不同的菌株质粒图谱一般不同。聚类分析表明:30株大肠杆菌遗传距离在0 224～0 416处聚成五类,其中同一地区聚为一类。这表明同一地区的大肠杆菌病的病原之间有一定的同源性。其次,这30株大肠杆菌在遗传距离为0 864处聚成一大类,说明它们之间有一定程度的遗传相关性。

To study antimicrobial susceptibility and biological characters of isolates of E coli from chickens, 30 isolates from different districts were tested. On the basis of antimicrobial susceptibility test,their antibiotic plasmids profile analyses were carried out The results will supply foundation for molecular epidemiological research of E coli from chicken The results of antimicrobial susceptibility test showed that the rate of drug resistance to ampicillin, penicillin,and tetracycline is 100.00%,that to chloromycetin,gentamycin,rifampin,and erythromycin is 6000%～9333%,and that of multiple drug resistance is 2667%～8000%. At 0224～0416 and 0864, all the strains clustered under 5 types. The results indicated that different sources of isolates showed different plasmid pattern types 