棉铃虫成虫对性信息素的电生理和行为反应研究

Electrophysiological and behavioral reponses of both sexes of the cotton bollworm, Helicoverpa armigera Hünber to sex pheromones

通过EAG和风洞实验 ,研究了棉铃虫雌雄成虫对性信息素组分和诱芯 (Z 11 16Ald∶Z 9 16Ald =97∶3)的电生理反应。其中棉铃虫雌、雄蛾对诱芯的平均EAG反应测定值分别为 1 0 6mV和 4 32mV ,分别高出对照 (无性信息素空白诱芯 )0 6 7mV和 0 6 6mV ,差异均达到极显著水平 (雌蛾 :t=2 5 0 2 0 ,P≤ 0 0 1;雄蛾 :t=44 2 6 9,P≤ 0 0 1) ;棉铃虫雌蛾对性信息素组分 (Z 11 16Ald和Z 9 16Ald)的EAG反应值随浓度增加而增加 ;雄蛾在被剪除触角后与雌蛾不能正常交配 ,而雌蛾在被剪除触角后仍有 40 %的交配率 ,比正常雌雄蛾的交配率 ( 70 % )有所下降 ;在风洞实验中 ,雄蛾没有顺风远离诱源的飞行行为 ,趋向诱源的比率为 81 8% ,与对照有显著差异。研究表明性信息素组分对棉铃虫的交配活动有明显的影响。

Electroantennograms (EAGs) were recorded for male and female moths of the cotton bollworm, Helicoverpa armigera Hünber. Lures impregnated with the sex pheromones (Z-11-16Ald∶Z-9-16Ald=97∶3) elicited greater EAGs from the antenna than the controls. EAG levels of females and males were 1.06?mV and 4.32?mV for the sex lures, and 0.67?mV and 0.66?mV for the control lures, respectively. There were significant differences between the sex lures and controls (female:t=25.020, P≤0.01; male:t=44.269, P≤0.01). The average EAGs of female moths increased with the concentration of the sex pheromones in the lures. Males that had their antennae removed were incapable of mating with normal females, while females that had their antennae removed had a mating success of 40% compared to 70% in normal females. In wind-tunnel trials male moths flew toward the sex lure at a rate of 81.8% and no moths flew away from the sex lure.