南方玉米锈病抗病基因的定位及不同遗传背景对基因标记的比较分析

Genetic Mapping of Resistant Gene to Southern Corn Rust and the Tagging Analysis on Different Genetic Background

玉米自交系齐 319高抗南方玉米锈病。利用SSR标记技术和BSA分析对齐 319抗南方玉米锈病基因进行了标记分析 ,结果表明SSR标记phi0 4 1和phi118与齐 319抗南方锈病基因连锁 ,其遗传距离分别为 7 6 9cM和 8 5 5cM。因此南方玉米锈病抗病基因定位于玉米 10号染色体短臂上。本研究进行的抗病基因标记 ,选择使用了两个杂交组合的 3个分离群体 ,标记结果显示同一杂交组合的不同分离群体其标记结果是一致的 ,而不同组合分离群体的标记结果有显著差异 ,这可能与基因的遗传背景有关。因此 ,在进行基因标记分析时 ,选择合适的分离群体是至关重要的。

Southern corn rust (SCR) is a destructive disease in maize.The inbred line Qi319 is highly resistant to southern corn rust.SSR technique was employed to preliminary mapping of the resistance gene.Bulked segregant analysis revealed that two primers,phi118 and phi 041,amplified polymorphic bands.SSR analysis on populations indicated the two primers were linked to the rust resistance gene,which was mapped on the short arm of chromosome 10.In addition,comparative analysis of the amplification bands among different populations revealed that the amplification products with the same primer in different populations were dissimilar.This result indicates that the genetic background may affect results of gene mapping and tagging.So,it is important to select suitable population to performing molecular marker analysis and gene mapping.