泡桐丛枝病发生相关蛋白质的电泳分析

SDS-PAGE OF PROTEINS RELATED TO PAULOWNIA WITCHE'S BROOM

对毛泡桐和白花泡桐同龄、同方位的病株健叶、病株病叶和健株健叶蛋白质进行了单向和双向SDS聚丙烯酰胺凝胶电泳分析研究。单向电泳结果表明 ,毛泡桐和白花泡桐病株健叶、病株病叶和健株健叶的蛋白质在种类和数量上存在一定的差异 ,其明显的蛋白质凝胶扫描谱带分别有 2 2、2 0和 1 7以及 2 7、2 1和 2 2条 ;双向电泳结果表明 ,毛泡桐与白花泡桐在健株健叶、病株健叶和病株病叶蛋白质变化方面具有一定相似性 ,即在两种泡桐健株健叶和病株健叶中存在的一种pI6.8、MW2 4KD蛋白多肽在病株病叶中观察不到。

Proteins in healthy and infected leaves of diseased trees and healthy leaves of healthy trees of Paulownia tomentosa Steud and P.Fortunei(Seem)Hemsl,which were on the same direction and at the same age,related to Paulownia witche's broom were studied by SDS-PAGE and 2-D-PAGE.The results indicated that there existed difference among healthy and infected leaves of diseased trees and healthy tree leaves of P.tomentosa and P.Fortunei trees in protein quantity and quality,in which obvious protein numbers were 22,20,17 and 27,21,22 with SDS-PAGE respectively.Moreover,the changes of proteins in healthy and infected leaves of diseased trees and healthy tree leaves of P.tomentosa had similarity to that of P.Fortunei on basis of 2-D-PAGE.That is,the protein(pI?6.8,MW24KD)existed in healthy tree leaves and infected-healthy leaves of diseased trees disappeared in diseased leaves.This protein change might be considered to have relationship with Paulownia witche's broom.