软肝冲剂对肝硬变过程中细胞周期DNA含量的影响

Effect of Ruangan Granule on DNA Content in Cellular Cycle of the Experimental Liver Cirrhotic Progression

目的 :研究软肝冲剂对 CCl4 肝硬变过程中各期细胞周期 DNA含量的影响 ,从分子水平探讨其促进肝细胞再生、阻止肝纤维化、肝硬变形成的作用机制。方法 :采用流式细胞仪检测 CCl4 肝硬变大鼠各期细胞周期DNA含量。结果 :定量 DNA图像分析发现模型组中处于 G1 期的细胞数明显多于其他各组 (均 P <0 .0 1) ,软肝冲剂大剂量组中处于 G1 期的细胞数明显少于小剂量组 (在实验的第 12、 4 5、90天 ,均 P <0 .0 1)。结论 :软肝冲剂能调节 CCl4 肝硬变过程中细胞周期各时相 DNA含量 ,促进肝细胞再生 ,抑制肝纤维化、肝硬变的形成。

Objective: To study the influence of Ruangan granule (RG) on DNA content in different phases of liver cirrhotic progression. Methods: The content of DNA of the hepatocytes of different phases was detected with Flow Cytometric Analysis.Results: Percentage of cells in G 1 phase of model group was much higher than that of the other groups (P<0.01). The DNA content of the large dose group in different phases was lower than that of the small dose group (P<0.01).Conclusion: RG has definite effect on hepatocyte proliferation. Its effect was better than that of colchicine treatment group. The possible mechanism was to regulate the cellular cycle, protect hepatocytes, improve the function of liver and resist the formation of liver cirrhosis.