蛇床子素对新生大鼠颅盖骨成骨细胞功能的调节作用

Regulatory effect of osthole on function of osteoblast in neonatal calvaria cultures

目的 研究蛇床子素对成骨细胞功能的影响。方法 原代培养新生大鼠颅盖骨成骨细胞 ,3H 胸腺嘧啶掺入法测定成骨细胞的增殖 ;Griess试剂法测定培养上清中NO的含量 ;小鼠胸腺细胞增殖法测定IL 1的含量 ;B9细胞增殖法测定IL 6的含量 ;RT PCR法检测IL 6的mRNA的表达。结果 蛇床子素抑制成骨细胞自发地或在炎性细胞因子及LPS刺激下产生NO、IL 1及IL 6和IL 6mRNA表达。结论 蛇床子素通过减少NO、IL 1和IL

AIM To study the regulatory effects of osthole on function of osteoblast. METHODS Osteoblastic cells were isolated from newborn rat calvaria through trypsin digestion. The proliferation of osteoblasts was assayed by 3H TdR.Levels of NO, IL 1 and IL 6 in the supernatant of rat osteoblast were determined by Griess agents, examing proliferation of thymic cells and bioassay B 9 cells as target cells. RT PCR analysis was used to determine the expression of IL 6 mRNA by osteoblast. RESULTS Osthole significantly inhibited the production of NO, IL 1 and IL 6 by osteoblast spontanously or induced by cytokines and LPS from osteoblast. It also inhibited the expression by IL 6 mRNA by osteoblast. CONCLUSION Osthole could regulate the function of osteoblast by reducing the production of NO, IL 1 and IL 6.