内皮细胞细胞外基质改性钛表面内皮细胞和血小板的生长行为

Endothelial Cells and Platelets Behavior on Titanium Modified by the Extracellular Matrix

实验结合碱活化和钛表面原位构建人脐静脉内皮细胞(HUVECs)细胞外基质(extracellular matrix,ECM)的方法制备细胞外基质改性的粗糙钛片(Ti-OH-ECM),然后比较粗糙钛(Ti-OH)表面和光滑钛(Ti,对照组)表面构建ECM前后的表面形貌、亲疏水性变化、官能团表征、细胞相容性和血液相容性。结果表明,ECM在钛表面构建成功,粗糙钛表面接触角降低至9°,ECM改性后,粗糙钛片的接触角有显著的增高,具有统计学意义(P<0.01);并且ECM在粗糙钛表面沉积的量显著大于对照组表面(P<0.01);1 d和3 d的细胞荧光染色结果表明,粗糙钛表面抑制内皮细胞黏附,但是ECM改性后的粗糙钛表面内皮细胞黏附数量高于对照组改性后的表面,并且具有统计学意义(P<0.05);与粗糙钛表面比较,ECM改性后的粗糙钛促进平滑肌细胞粘附及增殖;另外,2 h的血小板黏附结果表明,与对照组相比,粗糙钛的血小板黏附数量显著减少(P<0.01),ECM改性后的粗糙钛表面进一步抑制血小板黏附及激活(P<0.05)。因此,细胞外基质改性的碱活化钛为生物医疗器械提供了一定的研究基础。

HUVECs were cultured on flat Ti and alkaline activated Ti. After stripping off the cells,HUVECs derived ECM are deposited. Water contact angles,scanning electron microscope( SEM),FTIR have confirmed the successful deposition of ECM on Ti and alkaline activated Ti. ECM secretion of alkaline activated Ti is higher than Ti surface( P < 0. 01). To evaluate the hemocompatibility of the modified samples,adhesion and activation of platelets,attachment,adhesion,and proliferation of HUVECs have been carried out. The changes of surface topography shows ECM are coated on. The contact angle of Ti- OH decrease to 9°. After ECM coated on,the contact angle of Ti- OH-ECM increased significantly. In addition,the platelets adhesion and activation are inhibited on ECM-covered alkaline activated Ti( P < 0. 05) and ECs shows better proliferation ability on ECM-covered alkaline activated Ti( P < 0. 05). Therefore,the alkaline activated Ti provides a platform for ECM secreted from HUVECs,suggesting endothelial potential of their application in biomedical devices.