山茱萸多糖对衰老小鼠肝组织中SIRT1基因表达的影响

To study the effects and possible mechanism of Fructus Corni Polysaccharides on SIRT1 gene of aging model rats

目的:观察山茱萸多糖对D-半乳糖致衰老小鼠肝脏组织中SIRT1基因的表达的影响,探讨山茱萸多糖的抗衰老作用。方法:Wistar小鼠40只,雌雄各半,2~3月龄,体重18~22g,随机分为青年对照组、衰老模型组、山茱萸多糖小剂量组和山茱萸多糖大剂量组,每组10只。正常饮食水,衰老模型组及山茱萸大小剂量组每日上午颈背部注射D-gal(48mg/kg),连续45d,造模成功后衰老模型组经胃给予相当于给药组药量体积的温开水连续30d;大小剂量给药每日分别按0.4g/kg和0.2g/kg体重经胃给予山茱萸多糖。连续30d,后于末次给药后次日处死小鼠,取肝组织,RT-PCR法检测小鼠肝脏组织中SIRT1mRNA的表达,Western bloting法检测SIRT1蛋白的表达。结果:与青年对照组相比,衰老模型组SIRT1mRNA及蛋白质表达下降(P<0.01);衰老模型组比较,大小剂量给药组SIRT1mRNA及蛋白质升高,但差异不显著(P>0.05)。结论:山茱萸多糖能提高SIRT1mRNA及蛋白的表达,延缓衰老过程。

Objective:To study the effect and possible mechanism of Fructus Corni Polysaccharides(FCP)on the SIRT1 gene of aging model rats.Methods:Wistar rats were divided into young control group,aging group,large dose of Fructus Corni Polysaccharides group,and small dose of Fructus Corni Polysaccharides,with ten rats in each group.Except rats in control group,those in other groups were injected D-gal(48mg·kg-1·d-1)on napes for 45 days.Rats in aging group were perfused with warm water,and those in different dose groups were perfused with 0.4g/kg and 0.2g/kg FCP for 30 days.Then all rats were killed,and RT-PCR method was used to examine the mRNA expression of SIRT1.The effects of FCP on the above-mentioned indices were observed as soon as possible.Results:Compared with young control group,the expression level of SIRT1 mRNA in aging group was decreased(P <0.01).FCP could decrease the mRNA expression level of SIRT1(P >0.05).There is no difference between large and small dose FCP groups(P >0.05).Conclusions:FCP plays certain role in delaying aging because it can increase SIRT1 gene expression.