黄芪与雷米普利联合应用对UUO大鼠肾脏VEGF表达的影响

The combination effects of astragalus and ramipril on the expression of vascular endothelial growth factor in unilateral ureteral obstruction(UUO)rats

目的:探讨黄芪与雷米普利联合应用对单侧输尿管梗阻大鼠(UUO)肾脏血管内皮生长因子(VEGF)表达的影响。方法:45只雄性Wistar大鼠随机分为5组:单侧输尿管梗阻(UUO)组;对照组,除不结扎离断输尿管外,其余处理同UUO组;黄芪组,给予UUO模型大鼠黄芪注射液5mL/kg/d(相当于黄芪生药10g/kg·d)灌胃,(黄芪注射液由珍宝岛药业提供,批号A20120920,每支10mL,含生药20g);雷米普利组,给予雷米普利灌胃(10mg/kg·d);黄芪、雷米普利联合组,给予黄芪注射液(5mL/kg·d)、雷米普利(10mg/kg·d)灌胃。观察第14天肾间质纤维化程度、VEGF的表达。结果:1与假手术组相比,UUO组大鼠肾间质蓝染面积显著增加(P<0.05)。2与对照组相比,UUO组VEGF阳性面积百分比显著降低(P<0.05),黄芪、雷米普利和黄芪、雷米普利联合组的VEGF阳性面积百分比则显著增加(P均<0.05)。黄芪、雷米普利联合组与黄芪组、雷米普利组的差异均有统计学意义(P均<0.05)。结论:与单用黄芪、雷米普利相比,两药联合应用能更大程度的减轻UUO大鼠肾间质纤维化,增加VEGF表达可能是其机制之一。

Objective:To study the combination effects of Astragalus and Ramipril on the expression of vascular endothelial growth factor(VEGF)in Unilateral ureteral obstruction(UUO)rats.Methods:45male Wistar rats(weight 100±20g)were divided into 5groups:UUO group,Sham group,Astragalus treated UUO group,Ramipril treated UUO group and Astragalus plus Ramipril treated UUO group.Astragalus and/or Ramipril were administrated for 14 days.The expression level of VEGF in kidney tissue was detected by mmunohistochemical method.Results:1The area of renal interstitial fibrosis in UUO group is larger than that in sham group(P<0.05).2The VEGF positive area decrease in UUO group(P<0.05)and increased in treated groups(P <0.05).There are significant differences among these groups(P <0.05).Conclusion:Compared with Astragalus or Ramipril treatment,combination of Astragalus and Ramipril can relieve renal interstitial fibrosis.Upregulation of VEGF may be involved in its mechanism.