2型糖尿病肾病患者血清toll样受体4及髓样分化蛋白-2与其相关性研究

The study of the correlation between type 2 diabetic nephropathy toll-like receptor 4 and myeloid differentiation protein-2 levels

目的:研究2型糖尿病肾病患者血清toll样受体4(TLR4)与髓样分化蛋白-2(MD-2)在肾脏损害不同阶段的表达水平,初步探讨两者与2型糖尿病肾病的关系。方法:采用酶联免疫法检测TLR4及MD-2在健康者与2型糖尿病肾病患者血清中的水平变化,同时测定空腹血糖(FPG)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、血尿素氮(BUN)、血肌酐(SCr),即时尿标本的白蛋白、尿肌酐。结果:4组受检者血清TLR4和MD-2的水平两两比较,差异均有统计学意义(P<0.01)。TLR4和MD-2分别与病程、FPG、TG、TC、LDL-C、BUN、SCr、UACR、尿白蛋白呈正相关(P<0.01),与HDL-C、尿肌酐呈负相关(P<0.01)。结论:2型糖尿病肾病患者血清TLR4与MD-2显著升高,并参与了2型糖尿病肾病的发生及发展。

Objective:To investigate the expression of serum toll-like receptor-4(TLR4)and myeloid differentiated protein-2(MD-2),in type 2diabetic nephropathy during different stage of kidney impairment.The relationship between these two values,and the relationship between the two values and type 2diabetic nephropathy will be studied in this report.Methods:The values of TLR-4and MD-2were detected by enzyme-linked immunosorbent assay.Further,fasting plasma glucose(FPG),triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),blood urea nitrogen(BUN),serum creatinine(SCr),urinary albumin,urine creatinine were determinated at the same time.Results:The difference of TLR-4and MD-2in the four groups of patient were significant(P < 0.01).TLR-4and MD-2were positively correlated with the course of disease,FPG,TG,TC,LDL-C,BUN,SCr,UACR,and urinary albumin(P <0.01),while negatively correlated with the HDL-C and urine creatinine(P<0.01).Conclusion:The values of TLR-4and MD-2in blood serum of type 2diabetic nephropathy patients were increased significantly.And TLR-4and MD-2participate in the development of type 2diabetic nephropathy.