前列腺癌组织中SOX7及SOX9基因的表达及意义

The expression of prostate cancer SOX7 and SOX9 gene and its significance

目的:探讨前列腺癌组织中SOX7及SOX9的表达及意义。方法:采用免疫组化法对SOX7及SOX9在30例前列腺癌组织及30例前列腺增生组织中的表达差异进行验证,再采用反转录实时定量PCR(RT-QPCR)检测SOX7及SOX9mRNA在30例前列腺癌组织及10例前列腺增生组织中的相对定量差异。结果:实验组中的SOX7呈现了低表达,而在对照组中SOX7的表达偏高(P<0.05);SOX9在前列腺癌组织中呈现高表达,在前列腺增生组织中呈现低表达(P<0.05)。在前列腺癌组织中,SOX7的Ct值相对SOX9偏高,说明前列腺癌组织中SOX7的表达量相对于SOX9低。相比对照组,SOX7在前列腺癌组织中的相对定量偏低,而SOX9在前列腺癌组织中的相对定量偏高。结论:SOX7与SOX9的表达呈负相关。SOX9的表达与前列腺癌恶性程度正相关,SOX7的表达与前列腺癌恶性程度负相关。它们的表达与年龄无明显相关。

Objective: To discuss prostate cancer( prostate cancer,PCa) tissue expression and the significance of SOX7 and SOX9 mRNA. Method: Immunohisto chemical method was used to stain the differences of SOX7 and SOX9 expression differences in prostate cancer tissues and 30 cases of benign prostatic hyperplasia verify. Then quantitative real- time reverse transcription PCR( RT- QPCR) and SOX9 mRNA SOX7 were used to detect prostate cancer in 30 cases quantitative differences in the relative organization and 10 cases of benign prostatic hyperplasia. Result: SOX7 experimental group presented low expression,while in the control group,the expression of SOX7 was high( P < 0. 05). SOX9 was highly expressed in prostate cancer tissues,but showing low expression( P < 0. 05) in benign prostatic hyperplasia. In prostate cancer,compared with SOX7,Ct values of SOX7 was relatively high,indicating that the expression of SOX7 in prostate cancer was low compared with SOX9. Compared to the control group,relative quantification of SOX7 in prostate cancer tissues was low,and relative quantification of SOX9 in prostate cancer tissues was high. Conclusion: SOX7 and SOX9 expression is negatively correlated. SOX9 expression is positively related to the degree of malignancy of prostate cancer. The expression of prostate cancer is negatively correlated to malignancy SOX7. Their expressions were no significant correlation with age.