青春双歧杆菌的体外抑菌作用研究

Inhibitory effect in vitro of Bifidobacterium adolescentis on bacteria

目的:研究益生菌制剂来源的青春双歧杆菌对大肠埃希菌的体外抑菌作用。方法:将青春双歧杆菌与大肠埃希菌分别单独培养,测定不同培养时间的p H值和活菌数,然后将两菌进行混合培养,再测p H值和活菌数,并与单独培养时的结果进行比较。结果:琼脂平板打孔法显示青春双歧杆菌上清液有抑菌功能;共培养法显示青春双歧杆菌可抑制大肠埃希菌增殖的数量,而自身数量不受影响。结论:该益生菌制剂来源的青春双歧杆菌的酸性代谢产物是主要的抑菌物质。

Objective: To study the inhibitory effect in vitro of Bifidobacterium adolescentis coming from probiotic preparation on Escherichia. coli. Method: Bifidobacterium adolescentis and Escherichia. coli were singly cultured,respeclively. PH levels and viable bacteria amounts were determined at various time,then Bifidobacterium adolescentis were mixed cultured with Escherichia. coli. PH levels and viable bacteria amounts were determined,and the date obtained from the single and mixed cultured were compared. Results: Agar plate method of drilling hole shows that: Bifidobacterium adolescentis had inhibitory function; A total of culture method shows that:Bifidobacterium adolescentis had inhibitory effect on the proliferation number of Escherichia. coli. Conclusion: The acidity metabolin of Bifidobacterium adolescentis is the main Antibacterial material.