摘要
目的:观察SD大鼠成骨细胞在珊瑚表面的贴附、伸展及生长情况,并探讨珊瑚作为骨组织工程支架材料与细胞复合植入体内的最佳时间。方法:分离的骨髓基质干细胞用含10％胎牛血清的DMEM培养,5d后加矿化液诱导培养,分化为骨髓基质成骨细胞。将成骨细胞与处理过的珊瑚复合,通过细胞计数检测附着后的细胞生长增殖特性,并在扫描电镜下观察细胞在珊瑚表面的贴附情况。结果:细胞接种后,在材料表面贴附良好,继续增殖。结论:珊瑚的生物相容性良好,细胞贴附后继续保持成骨细胞的表型,作为骨组织工程的支架材料有较好应用前景。
AIM: To observe the adhesion and growth of SD rat osteoblast on the surface of coral and study the best time on coral combined with SD rat osteoblast in vivo. METHODS: Bone marrow stroma cells were cultured with DMEM containing 10% FBS. After inoculating the cells onto the surface of coral, we surveyed the characteristic of proliferation by cell counting. Using scanning electronic microscope, we observed cellular morphology. RESULTS: Cells could be attached to and extended on the surface of coral, and normally grown, proliferated. Coral could promote cell proliferation. CONCLUSION: The results show that coral has good biocompatibility. With rat bone marrow stroma cells, they can be used as biomaterials in bone tissue engineering.
出处
《中国临床康复》
CSCD
2003年第2期224-225,T003,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(30070818)
