土壤微生物总DNA的提取和纯化

Extraction and Purification of Soil Microbial Total DNA

本文建立了从土壤中提取总DNA的方法 ,并通过改进使适合于对革兰氏阳性菌的提取。用 9种性质不同的土壤进行验证 ,均提取到了DNA ,每克干土的DNA提取量从 3 30 μg～1 3 41 μg,通过透析袋回收进行纯化 ,纯化回收率达到 65 34% ,纯化后的土壤DNA可以直接扩增出 1 6SrDNA。 9种土壤的提取率从 60 5 1 %～ 93 45 % ,可以从每g干土添加

A method of extracting soil total DNA was developed, and it can extract DNA from G + bacteria. The soil total DNA was extracted from 9 different soils,the DNA yeild was from 3 30μg to 13 41μg. The crude soil DNA was purified by dialysis bag, the recovery rate is 65 34%。The extracted rate in the 9 soils was 60 51%～93 45%. The traget gene could be amplified from the soil which was added 362 target cell/g.dry soil.