摘要
以硝普钠 (SNP)、精胺 (Sper/NO)和亚硝基谷胱甘肽 (GSNO)作为一氧化氮 (NO)供体用于处理卵囊 ,探讨外源性NO对卵囊的孢子化率及其致病力的影响。试验结果表明 ,在以上 3种NO供体中 ,只有GSNO对卵囊的孢子化有明显的抑制作用 ,其对纯化卵囊的抑制率达 93%～ 97% ,对粪便中卵囊的抑制率可达 10 0 % ;GSNO还能明显抑制孢子化卵囊的致病力 ,经 2～ 8mmol·L-1浓度GSNO处理的卵囊明显丧失了对雏鸡的致病作用。
The effects of exogenous NO on Eimeria tenella (E. tenella) oocysts were studied. The E.tenella oocysts freshly isolated from feces of experimental chickens infected with E.tenella. The sporulated E.tenella oocysts were treated in vitro by the well known NO donors, SNP, Sper/NO and GSNO. The results showed that among the 3 NO donors, only GSNO had significant effects on the percentage of sporulated oocysts, the inhibiting rate of purified sporulated oocysts was 93%-97%, and reached 100% on the oocysts in the feces of chickens with E.tenella infection. GSNO also significantly decreased virulence of E.tenella oocysts, and the oocysts treated with 2～8 mmol·L -1 GSNO almost lost their pathogenicity to chickens.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第3期336-341,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目 ( 3 9970 5 64 )
