细胞因子诱导杀伤细胞与抗原特异性细胞因子诱导杀伤细胞杀伤肿瘤效应比较的实验研究

Experimental study on cytotoxic activity of antigen specific cytokine induced killer cells

目的 :制备具有抗原特异杀伤活性的效应细胞。方法 :反复冻融法提取肿瘤可溶性抗原 ,甲基噻唑基四唑 (methylthiazolylterrazolium ,MTT)法检测效应细胞杀伤活性。 结果 :经树突状细胞 (dendriticcell,DC)摄取肿瘤细胞抗原 ,并提呈给细胞因子诱导杀伤 (cytokineinducedkiller ,CIK)细胞所获得的DC A CIK细胞对同一肿瘤靶细胞的杀伤活性 ,较CIK细胞、未摄取抗原的DC CIK细胞及摄取不相同肿瘤抗原的DC A不相同 CIK细胞具有更高的杀伤活性。结论 :摄取肿瘤可溶性抗原的DC细胞可明显提高CIK细胞对相同肿瘤靶细胞的杀伤特异性和杀伤活性。

Objective: To prepare antigen specific cytokine induced killer(CIK) cells. Methods: Soluble antigen was prepared from tumor cells by repetitive freeze thaw method. Cell killing activity is measured by MTT. Results: Cytotoxic activity was compared among CIK cells derived under three different conditions. (1)CIK cells prepared in the presence of the same tumor antigen fed dendritic cells ( DC ); (2)CIK cells prepared in the presence of DC without feeding the same tumor antigen; (3)CIK cells derived in the absence of DC. Among the three groups tested, DC A CIK cells showed the highest cytotoxic activity. Conclusion: Soluble target tumor antigen fed DC can significantly enhance the specificity and cytotoxicity of CIK cells to tumor target cells.