小细胞肺癌免疫磁性微珠的构建及鉴定

Study of Seperating Tumor Cells from Peripheral Blood MononuclearCells with Immunomagnetic Beads

目的 :构建能特异地与靶细胞结合并赋有磁响应性的小细胞肺癌免疫磁性微珠 ,检验其在分离微量癌细胞中的效率。方法 :将微量小细胞肺癌H12 8细胞加入到外周血单个核细胞悬液中混匀 ,再通过特异性单抗包被的免疫磁性微珠吸附、富集、免疫细胞化学鉴定。最后 ,计算癌细胞的回收率和检测敏感性。结果 :包被的小细胞肺癌免疫磁性微珠与H12 8能敏感而特异地结合。与免疫细胞化学方法结合可检出 5 4 .4 %混入外周血单个核细胞中的微量癌细胞 ,未有假阳性。结论 :免疫磁性微珠能有效地从外周血单个核细胞中分离出微量肺癌细胞 。

Objective: To coat immunomagnetic beads of small cell lung cancer which can be specially combined with target cells and to test the efficiency of immunomagnetic beads. Methods: A model study was performed by mixing small numbers of cells from the small cell lung cancer line H128 with peripheral blood mononuclear cells. The rare cancer cells were enriched by immunomagnetic beads coated with specific antibody and identified by immunocytochemistry.The recovery rate of tumor cells and detecting efficiency of immunomagnetic cell seperation were caculated. Results: Immunomagnetic beads could be specially and sensitively combined with H128 cells.The 54.4% rare tumor cells were detected by the above methods and no false positive results were obtained. Conclusion: The coated immunomagnetic beads can efficiently separate rare lung cancer cells from peripheral blood mononuclear cell superatant, and may be important for clinical practice after further study.