哮喘患者血清ECP、IL-5、总IgE测定的临床意义

The clinical significance of determining ECP,IL-5 and total IgE contents in serum of asthma patients

目的 进一步探讨支气管哮喘的发病机理。方法 分别采用免疫荧光法和固相酶联免疫吸附法 ,测定 32例支气管哮喘发作期 ,30例缓解期以及 30例正常对照组血清中ECP、IL 5、总IgE水平。结果 急性发作期支气管哮喘患者的ECP、IL 5、总IgE含量分别为 19.6 8± 7.4 3(μg/L)、89.2± 2 5 .9(ng/L)、5 2 4 .6± 138.7(KU/L) ;缓解期为 5 .76± 4 .2 5 (μg/L)、17.5± 7.3(ng/L)、2 91.3± 85 .4 (KU/L) ;正常对照组为 4 .86± 2 .4 8(μg/L) ,15 .1± 5 .6 (ng/L) ,10 3.2± 31.5 (KU/L)。哮喘急性发作期ECP、IL 5、总IgE分别比缓解期和正常对照组显著升高 (P <0 .0 0 1) ;ECP、IL 5二项缓解期与正常对照组无显著差异 (P >0 .0 5 ) ;总IgE缓解期比正常对照组显著升高 (P <0 .0 0 1)。结论 血液中ECP、IL 5、IgE一起参与了支气管哮喘的发病过程 ,测定三者的水平对判断支气管哮喘病情的进展有着十分重要的价值。

Objective To further explore the mechanism of the bronchial asthma.Methods The ECP,IL 5 and total IgE contents in serum of thirty two breakout of bronchial asthmas,thirty in releaving,and thirty normal control group were determined by immune fluorescence technique and solid phase ELISA.Results The ECP,IL 5 and total IgE level in patients with bronchial asthma 19.68±7.43(μg/L).89.2±25.9(ng/L),524.6±138.7(KU/L)in acute breakout group;5.76±4.25(μg/L),17.5±7.3(ng/L);291.3±85.4(KU/L)in relief group;4.86±2.48(μg/L),15.1±5.6(ng/L),103.2±31.5(KU/L)in the control group.The ECP,IL 5 and total IgE contents in acute breakout group were significantly higher than those in the relief group and in the control group;the ECP and IL 5 level have no significant difference between the relief group and the control group;the total IgE contents in the relief group were significantly higher than that in the normal group.Conclusion The ECP,IL 5 and IgE in the blood are involved in the onset process of the bronchial asthma.It is of some value to determine their serum concentration for the purpose of judging the progress of the bronchial Asthma.