嗅神经鞘细胞移植修复成鼠半横断胸髓损伤

Repair of adult rat semitransected thoracic spinal cord by transplants of olfactory ensheathing cells

目的:观察嗅神经鞘细胞(olfactory ensheathing cells,OECs)移植对脊髓轴突再生及神经功能恢复的促进作用。方法:将分离、培养3W的SD大鼠OECs,移植于12只成年SD大鼠胸10水平左侧半横断脊髓两断端;12只对照动物只注入等量DMEM;移植后6个月行下肢功能评价、运动诱发电位(MEP)检测、组织学和免疫组化检查。结果:实验组及对照组动物存活率均为83.4％。实验组9只(9/10)动物下肢功能有不同程度的改善,其中3只可以主动攀登;上述9只动物左下肢可记录到MEP;组织学检查见宿主再生轴突长人断端间组织,周围有髓鞘碱性蛋白阳性物质存在。对照组中2只(2/10)下肢功能稍改善,并能记录到MEP;组织学检查见轴突变性,瘢痕形成,无轴突再生。结论:OECs日移植后可在脊髓中产生髓鞘样物质,促进宿主脊髓轴突再生及神经功能部分恢复。

AIM: To observe the effect of olfactory ensheathing cells (OECs) transplantation on spinal cord axonal regeneration and spinal functional improvement METHODS: 24 rats were divided into two groups, each group had 12 rats. OECs suspension, purified from SD rat olfactory bulb, was transplanted into the left semitransected spinal cord at T10 level, while the vehicle solution (DMEM) was injected in the control group. At 6 months post-transplantation, the rats were evaluated with climbing test and MEP monitoring. The samples were procured and studied with histologiesl and immunohistochemical methods. RESULTS: Both of the two groups had the same survival rate 83. 4%. In the transplanted group, 9 rats(9/10) had lower extremities' functional improvement, three of them could actively climb. Nine rats mentioned above could be recorded MEPs in their left legs. Morphology of transplanted OECs was normal, and OECs' arrangement is good in host Axons could regrow into gap tissue between the injured spinal cords. Both OECs and regrowed axons were immunoreactive for MBP. In the control group, 2 rats (2/9), with MEPs recorded, had lower extremities muscles' contraction. No regrowed axons could be seen except scar formation in the control group. CONCLUSION: Transplanted OECs could construct the myelin sheath and promote regrowing axons and functional improvement