增生性瘢痕中细胞外信号调节激酶及其激酶基因表达的变化

Gene expression of extracellular-signal regulated protein kinase1/2 and their MAPKKs in hypertrophic scars

目的 :探讨细胞外信号调节激酶 1/ 2 (extracellular signal regulated protein kinase,ERK1/ 2 )及其上游信号分子 MEK1/ 2在增生性瘢痕和正常皮肤中基因表达的变化。方法 :提取 8例增生性瘢痕和 8例正常皮肤组织的总 RNA后 ,分离 m RNA,用逆转录聚合酶链反应 (RT PCR)法检测这 4种基因在不同组织中的表达变化规律。结果 :在正常皮肤组织中 ,MEK1、MEK2、ERK1和 ERK2基因表达较弱 ;而在增生性瘢痕中 ,这 4种基因表达明显增强 ,其 m RNA量的灰度比分别为正常皮肤的 1.1倍、1.2倍、2 .2倍和 2 .5倍。结论 :增生性瘢痕的发生可能与 MEK1/ 2和 ERK1/ 2基因表达升高有关。

Objective:To explore the change of extracellularsignal regulated protein kinase1/2 (ERK1/2) and their mitogen activated protein kinase kinases(MAPKKs) gene expression in normal skins versus hypertrophic scars underling their biological significances.Methods:After total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skins,and then were purified to mRNAs, the gene expression of ERK1/2 and MEK1/2 were examined with reverse transcriptionpolymerase chain reaction (RTPCR). Results:In normal skins,the intensity of gene expression of ERK1/2 and MEK1/2 were weak .In hypertrophic scar,the expression intensities of these 4 genes were 1.1 times,1.2 times, 2.2 times and 2.5 times of normal skins,respectively.The expression of these 4 genes was significantly elevated in comparison with normal skins.Conclusion:The activation of MEK1/2,ERK1/2 gene expression in hypertrophic scars versus in normal skins might be one of the mechanisms controlling the formation of hypertrophic scars.