中华蜜蜂和意大利蜜蜂蜂毒前溶血肽原蛋白cDNA的克隆与序列分析

Cloning and sequencing of cDNA encoding prepromelittin in Apis cerana cerana and Apis mellifera

分别从中华蜜蜂Apisceranacerana和意大利蜜蜂Apismellifera工蜂毒腺中抽提总RNA ,通过RT PCR方法扩增 ,各得到了蜂毒前溶血肽原蛋白的cDNA ,再将扩增产物克隆到pGEM Teasy载体上 ,进行测序和序列分析。结果表明 ,所扩增到的这两个片段长度均为 2 13bp ,均为编码蜂毒前溶血肽原的cDNA ,并分别推导出两者所编码的氨基酸序列。经序列比较 ,中华蜜蜂前溶血肽原与意大利蜜蜂、印度蜜蜂Apisceranaindica前溶血肽原的同源性都为 97%。所报道的中华蜜蜂蜂毒前溶血肽原的核苷酸序列的GenBank登录号为AF48790 7。

Two cDNA fragments encoding prepromelittin were amplified by RT-PCR fr om the total RNA from the venom glands of the worker honeybees, Apis cerana cerana and A. mellifera. The PCR products were ligated into pGEM-T easy vector. The results of sequencing showed that the ORFs coding for prepromel ittin were all 213 bp in length. The amino acid sequence of A. cerana cerana prepromelitti n shared 97% homologies with that of A. mellifera prepromelittin and A. ce rana indica prepromelittin. The nucleotide sequence reported here was submitte d to GenBank with accession no. AF487907.