摘要
采用聚丙烯酰胺凝胶电泳法对10种早熟禾属植物过氧化物酶(POD)同工酶进行分析,结果表明:供试材料共出现了58条酶带,但每个种的带数有所不同,最多出现8条,最少只有4条;每种植物的POD酶谱大致可分为A、B、C三区,其中酶带数量最多、活性最强、分布较为集中的是B区;各供试植物在酶谱特征以及种间相似程度上均有明显差异,POD同工酶技术对早熟禾属植物进行物种鉴定以及种间遗传距离分析是可行的。
The POD isozyme for ten species of Poa were analyzed by polyacrylamide electrophoresis.The results showed:the experiment materials had 58 isozyme bands, but the number of isozyme bands was different with species,the maximum was 8 and the minimum was 4.The POD zymograms of each species can be divided into three regions(A,B,C) from negative pole to positive pole,in which the B region has more isozyme bands,strong activity degree of similarity in inter_species.Therefore, this method of POD isozyme analysis can be used to identify species of Poa and study genetic distance and relationships among the species.
出处
《中国草地》
CSCD
2003年第2期30-33,共4页
Grassland of China
基金
甘肃省列项目(QS-981-B31-024)资助
