流式细胞仪检测直肠癌患者外周血免疫细胞及临床意义

Immunodetctions of Patients with Rectum Cancer by Flow Cytometry and Its Clinical Significance

目的 :了解直肠癌患者免疫功能状态及术前术后的变化。方法 :用流式细胞仪检测直肠癌患者及术前术后外周血中CD+3、CD+4 、CD+8、CD+16 和CD+56 的淋巴细胞及γδΤ细胞数量及比例。结果 :CD+3、CD+4 、CD+8、γδΤ、CD+16 和CD+56 细胞检测结果直肠癌患者组分别为 5 0 .2 6± 13.62 %、2 7.2 0± 9.0 4%、2 9.0 6± 12 .84%、5 .12± 3.14 %、16.77± 8.71%和 17.2 6±9.45 % ;对照组为 72 .13± 10 .89%、39.5 3± 12 .10 %、2 8.3± 13.0 7%、5 .47± 2 .35 %、18.13± 6.35 %和 16.89± 7.13%。结论 :直肠癌患者免疫功能紊乱 ,细胞免疫功能明显低下 ,临床分期越晚 ,这种表现越明显 ,而术后患者免疫功能有一定改善。流式细胞仪测定上述多项免疫指标 。

Objective:In order to compare the immune conditions of the patients with rectum cancer at different clinical stages and the changes of immune conditions pre and post operations.Methods:Immunodetections were performed by FCM.The call counts and ratios of CD + 3?CD + 4?CD + 8?CD + 16 cells and γδΤ cells in the peripheral blood were tested.Results:The ratios of CD + 3?CD + 4?CD + 8?γδΤ?CD + 16 and CD + 56 in the patients with rectum cancer were 50.26±13.62%?27.20±9.04%?29.06±12.84%?5.12±3.14%?16.77±8.71% and 17.26±9.45% tespectivel;The tatios for contral group were 72.13±10.89%?39.53±12.10%?28.3±13.07%?5.47±2.35%?18.13±6.35% and 16.89±7.13% tespectivel.Conclusion:The immune conditions of patinets with rectum cancer were lower and in disorder,especially the cellular immunity.With the progress of the disease,the changes were more distinctive.The immune conditions had some improvement post operation.FCM analysis of immune conditions of patients with rectum cancer could have important clinical values in prognosis evaluation.