自身免疫病患者外周血CD34^+细胞动员、采集和纯化研究

Study on the peripheral blood CD 34^+ cell mobilization, harvesting and purification on patients with auto-immune diseases

目的 探讨自身免疫病患者外周血造血干细胞动员、采集和纯化的效果及安全性。方法 对17例确诊自身免疫病患者予环磷酰胺+粒细胞集落刺激因子(G-CSF)动员外周血干细胞,CliniMACS系统对其中11例患者外周血单个核细胞进行CD34+细胞阳性分选,流式细胞仪检测分选前后CD34+细胞纯度及淋巴细胞各亚群。结果 1例动员中并发尿崩症,2例动员失败。11例分选后CD34+细胞纯度平均96.1％,回收率70.14％;CD3+、CD 19+、CD 14+细胞分别去除103/kg、102/kg、103/kg。结论 对自身免疫病患者采用环磷酰胺+G-CSF方案动员外周血干细胞方法可靠,安全性好;外周血干细胞的纯化方法可获得高纯度和高回收率的CD34+细胞,并极大限度的去除T、B等细胞。

Objective To evaluate the efficacy and safety of the peripheral blood stem cell( PBSC) mobilization,harvesting and purification on patients with autoimmune diseases (AIDS). Methods Seventeen patients with AIDS were included in this study. The regimen for PBSC mobilization was cyclophosphamide +G-CSF. The CliniMACS CD34+ cell device was used for positive selection of 11 leukapheresis products. Purity of CD34+ cells and lymphocyte subsets was determined by FACS analysis on preseparation and postseparation samples. Results One case was complicated with diabetes in-sipidus during mobilization, 2 patients failed in mobilization in all the 17 AIDS patients. 11 separations were performed. About 70. 14% CD34+ cells were recovered with a median purity of 96. 1%. Depletion of CD3+ cells,CD19+ cells and CD14+ cells were 103/kg,102/kg and 103/kg respectively. Conclusion Cyclophosphamide + G-CSF is a safe and effective mobilization regimen for PBSC on AIDS patients. CD34+ cells with high purity and recovery can be isolated with concomitant effective T and B cell deletion with this purification method.