再生障碍性贫血患者外周血及骨髓sCD40L水平检测及意义

Detection of soluble CD40L level in peripheral blood and bone marrow of patients with aplastic anemia

目的 探讨可溶性CD40配体(sCD40L)在再生障碍性贫血(再障)患者体内的变化及其发病机制中的作用。方法 用ELISA方法检测40例再障患者及正常人外周血及骨髓中sCD40L水平;用免疫荧光标记技术和流式细胞仪分析患者骨髓及外周血淋巴细胞的表型。结果 再障患者外周血及骨髓sCD40L较正常人均显著降低(P均<0.001);患者可溶性CD40L水平与外周血粒细胞和血小板计数相关;患者骨髓及外周血CD4+细胞比例下降,CD4/CD8比值降低。结论 sCD40L降低可能是再障发病的重要因素之一,其外周血及骨髓水平的检测对明确诊断和判断预后有重要的临床意义,重组人可溶性CD40L的应用可能为再障的治疗开辟另一新的途径。

Objective To investigate the alteration of soluble CD40L level in patients with aplastic anemia(AA) and its possible role in the pathogenesis of AA, Methods Peripheral blood and bone marrow level of soluble CD40L in 40 AA patients and 20 normal controls was detected by enzyme linked immunosorbent assay (ELISA); Lymphocyte phenotype was analyzed by immunofluorescence and flow cytometry. Results The levels of sCD40L in peripheral blood and bone marrow both decreased signifieantly (P<0. 001) ;the sCD40L level of AA was related to the count of peripheral WBC and platelet; the percentage of CD4+T cells decreased and the ratio of CD4/CD8 decreased. Conclusion The decreased level of sCD40L may be the potential reason of AA,detection of peripheral blood and bone marrow may be helpful for the diagnosis of AA and the application of CD40L will probably be an new method for the therapy of AA.