人胆管癌裸鼠移植瘤模型的建立

Establishment of Implanted Tumor Models of Human Bile Duct Carcinoma in the Nude Mice.

目的:建立人胆管癌裸鼠移植瘤1号和2号模型。方法:将人胆管癌组织接种于裸鼠皮下和肝脏,逐代观察移植瘤的生长情况,绘制其生长曲线,进行形态学和生物学特性鉴定。结果:建立了人胆管高分化粘液腺癌裸鼠移植瘤1号和中分化乳头状腺癌裸鼠移植瘤2号模型。皮下移植瘤生长率为40%;1、2号模型移植成功率分别为97.7%和100%,潜伏期分别为26d和217d。移植瘤在形态和生物学上仍保持人胆管癌的特点。结论:裸鼠移植瘤1、2号模型是一种接近人体的胆管癌模型,可为胆管癌研究提供实验平台。

Objective:To establish the implanted tumor models of human bile duct carcinoma in the nude mice.Methods:Surgical specimens of patients with bile duct carcinoma were implanted into the subcutaneous layer and liver of nude mice.Growth lines of implanted tumors were drawn and its morphological and biological characteristics observed.Results:A well differentiated gelatinous adenocarcinoma model and a moderately differentiated papillary adenocarcinoma model of human bile duct carcinomas in nude mice were established and named HBDCM1-ZSH and HBDCM2-ZSH fespectively.The growth rate of subcutaneous implantation was40%;implanted tumors of model1and model2tumors exhibited97.7%and100%implantability in the nude mice,their latent periods were26d and21d respectively and exhibited the characteristics of the original tumors both morphologically and biologically.Conclusion:Models HBDCM1-ZSH and HBDCM2-ZSH resemble to their original human bile duct carcinoma types and provide good animal models for bile duct carcinoma research.