薄层扫描法测定抗心毒颗粒中黄芪甲苷的含量

Quality standard for KANG XINGDU Granule by TLC

目的 :建立用薄层扫描法测定抗心毒颗粒中黄芪甲苷含量的方法。方法 :甲醇提取 ,乙醚脱脂 ,正丁醇萃取后蒸干 ,上D10 1型大孔树脂柱洗脱 ;以 [正丁醇 -醋酸乙酯 -水 (4∶1∶5 )的上层液 ]-甲醇 (10∶1)为展开剂 ,置氨蒸汽饱和的展开缸内 ,喷以 10 %硫酸乙醇溶液 ,10 0℃至斑点显色。检测波长 :λs =5 30nm ,λr =70 0nm ,狭缝 2× 1.2 ,线性参数Sx=3。结果 :3批样品中黄芪甲苷的平均含量为 0 .5 2 0mg/袋 ,平均回收率为 96 .90 %。结论 :上述方法简单、准确 。

Objective: To establish the quality standard for KANG XINGDU Granule. Methods: Astragaloside of KANG XINGDU Granule was determined by TLC-scanning. Results: Astragaloside I showed a good relationship linear of 0.111-0.555ug and the average recovery was up to 96.90%, and RSD was 1.37%. TLC spot develops were fairly clear, and the blank test showed no interference. Conclusion: The methods can be used for the quality control of the capsule.