摘要
目的 探讨青年人原发性肺癌组织细胞中 ,凋亡相关基因bcl- 2及bax表达水平及其与临床预后的关系。方法 采用免疫组织化学法 ,分别检测 5 2例青年人肺癌组织中bcl- 2、bax的表达情况。结果 在 5 2例肺癌组织中有 2 3例 (44 .2 % )bcl- 2蛋白表达阳性 ,其中 3例小细胞肺癌全部阳性。在非小细胞肺癌中bcl- 2蛋白呈过度表达 ,低分化癌明显高于高、中分化肺癌 (P <0 .0 1)。有 2 7例 (5 1.9% )肺癌组织bax表达阳性中 ,Ⅰ期肺癌占 82 .4 % (14例 ) ,Ⅱ期肺癌占 (71.4 % ) 10例 ,明显高于Ⅲ期肺癌的 (14 .3% ) 3例。高、中分化肺癌的bax表达显著高于低分化肺癌 (P <0 .0 5 )。结论 bcl- 2蛋白在低分化肺癌中呈过度表达 ,bax在早期和高中分化肺癌中呈高表达 ,它们分别在肺癌发生发展过程中不同途径上抑制肺癌细胞的凋亡 ,对肺癌早期诊断和判断预后有一定的意义。
Objective To investigate the relationship between expression of bcl-2, bax in lang cancer in young patients and their prognostic evaluation. Methods Expression of bcl-2 protein and bax in 52 cases of surgically resected lung were investigated respectively by immunohistochemical techniques. Results The positive espression of bcl-2 protein was observed in 44.2%(23/52) cases, in which 3 cases of small-cell lung cancer were all positive. For non-small cell lung cancer, the bcl-2 expression rate was much higher in those with poor differentiation than those with well differentiation (P<0.01). The expression of bax was detected in 51.9%(27/52) cases, in which the expression rate was much higher in stage I, II than in stage III disease (82.4%, 71.4% vs 14.3%,P<0.05). Conclusions The expression of bcl-2 protein is predominately observed in lung cancer, at late while expression bax at early stage.They inhibit apoptosis of tumor cells on the different way during the pathogenesis of lung cancer, respectively. They play a certain role in evaluating prognosis and early diagnosis of pulmonary carcinoma.
