洛沙坦对兔自体静脉移植物平滑肌细胞增殖的影响

Effect of Losartan on proliferation of cell nuclear antigen of venous autograft

目的 探讨洛沙坦对自体静脉移植物平滑肌细胞增殖的影响 ,寻找防止静脉移植物内膜增生的合理、有效的途径。方法 4 8只新西兰大白兔随机分为治疗组和对照组 ,每组各 2 4只 ,分别于自体静脉移植术后1、3、5、7d ,每组处死 6只动物 ,取移植物行免疫组织化学染色 ,对增殖细胞核抗原阳性 (PCNA)的平滑肌细胞进行定性、定量观察。结果 术后第 1天PCNA即呈阳性反应。术后第 5天细胞增殖达到高峰 ,对照组PCNA阳性细胞数为 2 4 31± 4 0 9,明显高于治疗组 (1372± 2 6 3) ,两者相差异有显著性 (P <0 .0 1) ;对照组增殖指数为 89± 3,显著高于治疗组 (5 1± 4 ,P <0 .0 1)。结论 洛沙坦可抑制自体静脉移植物的平滑肌细胞增殖 ,对预防冠状动脉旁路移植术后静脉桥狭窄可能有一定的临床意义。

Objective To study the effect of Losartan on proliferation of cell nuclear antigen of venous autograft in order to find an effective way to prevent intimal hyperplasia. Methods 48 New Zealand white rabbits were randomly divided into 2 groups, treatment group and control group with 24 for each. External jugular vein of New Zealand rabbits were harvested and grafted into the ipsilateral carotid artery. After operation,the rabbits were fed with Losartan (25 mg·kg -1 ·d -1 in treatment group) and physiological saline (2.5 ml·kg -1 ·d -1 in control group) through drinking water. At the first, third, fifth, and seventh day post operatively , 6 rabbits were killed in each group, and 5 μm sections of the veins were stained with reagent box for proliferating cell nuclear antigen(PCNA). Results At the first day after operation, positive cells with PCNA were found and reached the peak on the fifth day, the number of positive PCNA were 1372±263 and 2431±409 for treatment and control group respectively. The difference of PCNA between the two groups was of statistical significance ( P <0.01). Conclusion Losartan can restrain the smooth muscular cell of venous autograft from proliferation and may have some significance in preventing bypass graft from stenosis after coronary artery bypass graft (CABG).