妊娠高血压综合征与组织相容性白细胞抗原-E的关联研究

Study on association of pregnancy-induced hypertension with HLA-E polymorphism

目的 探索妊娠高血压综合征 (PIH)的发生与组织相容性白细胞抗原 (HLA) E基因多态性是否存在关联。方法 采用地高辛标记的寡核苷酸探针杂交技术 ,检测 5 8例PIH患者 (实验组 )和 15 6名正常孕妇(对照组 )的HLA E等位基因多态性。结果 实验组和对照组中 ,HLA E 0 10 1均为最常见的等位基因 ,其等位基因频率分别为 4 3.1%和 4 2 .2 5 % ;其次是E 0 10 32 ,分别为 2 8.4 5 %和 32 .84 % ;E 0 10 31分别为 2 8.4 5 %和2 4 .88% ;HLA E 0 10 2和E 0 10 4在两组标本中均未检出。两组间检出的 3个等位基因的差异均无显著性 (P>0 .0 5 )。结论 PIH的发生与HLA E等位基因多态性可能无直接关联。

Objective To explore the possible association between the pathogenesis of pregnancy induced hypertension (PIH) and the histocompatibility leukocyte antigen (HLA) E gene polymorphism. Methods The frequencies of five HLA E alleles in 58 women with PIH and 156 random normal pregnant women in Shanghai area as controls were investigated. PCR sequence specific oligonucleotide (PCR SSO) typing method was used to detect the polymorphism in exon2 and exon3 of HLA E gene.Results E*0101 was the commonest allele in both PIH patients and controls (43.10% and 42.25% respectively). E*01031 and E*01032 were also detected with frequencies of 24.88% and 32.84% in controls, 28.45% and 28.45% in PIHs respectively. No HLA E*0102 nor E*0104 alleles could be detected in both groups. No significant difference between both groups were observed. Conclusion It is suggested that maternal HLA E polymorphism may not be associated with the pathogenesis of PIH and further investigation is mandatory.