人参皂甙Rb_1、Rb_3、Rg_1对培养小鼠皮层细胞缺血损伤的保护作用及浓度—效应关系

Study Of protective effects of Ginsenosides Rb1、Rb3、Rg1 on ischemic injuyies in Cultured mouse cortical neurons and the relationship of concentration and effect

目的:研究人参皂甙(Ginsenosides,Gs)的三种单体成分GSRb_1、GSRb_3、GSRg_1对培养小鼠皮层神经细胞缺血损伤的保护作用。方法:①将离体培养的ICR小鼠胎鼠神经细胞缺血培养以模仿缺血对中枢神经元的损伤,观察不同缺血时间神经元活性变化。②利用MTT比色法观察不同终浓度(0、20、40、60、80、100μmol/L)的GS Rb_1、Rb_3、Rg_1对缺血神经细胞的作用。③检测细胞外液LDH释放量以观察60μmol/L浓度的GSRb_1、Rb_3、Rg_1对缺血神经细胞存活的影响。结果:①培养的小鼠胎鼠皮层神经元随着缺血时间的延长,神经元活性逐渐降低,缺血时间越长,神经元活性下降越多、细胞受损越明显、死亡率增加。②在所研究GS浓度范围(20～60μmol/L)中,对神经细胞的保护作用呈现浓度依赖性,以60μmol/L终浓度的GS单体能明显提高缺血神经元的活性和生存能力,减轻细胞的形态学损伤,且这种保护作用以GSRb_2最明显,随着浓度的再进一步升高,保护作用减弱,100μmol/L的GS单体对缺血神经元无保护作用。结论:适宜浓度人参皂甙单体对缺血培养的小鼠胎鼠皮层神经细胞具保护作用。

Objective: To study the neruoprotective effects of three monomers of Ginsenosides (GSRb1、GSRb3、GSRg1 ) on ischemic injuries in cultured mouse cortical neurons Methods: ①MTT assay was used to compare the effects of different duration of ischemia on the cultured mouse cortical neurons. ②Various concentrations of GSRb1、Rb3、Rg1 (20-100μmol/L)were used to observe their effects on ischemic cultured mouse cortical neurons and neuronal viabilities in primary cultures from mouse cerebral cortex assessed by means of MTT assay.③The measurement of the release of LDH and morphological observation were used to research the effects of GS (60μmol/L) on neuronal death. Results: ① The neuronal viability decreased or the cell death rate increased as ischemia duration prolonged. ②GSRb1、Rb3、Rg1 could improve the neuronal viability respectively in a dose dependent manner in the range of 20 to 60μmol/L. These effects were most significant at the concentration 60μmol/L. While in the range of 60 to 100μmol/L. the protective effects of GS decreased. GSRb1、 Rb3、 Rg1 at 100μmol/L had no protective effects. Conclusion: Certain concentration GSRb1、Rb3、Rg1 have affirmative protective effects on ischemia induced neuronal damage.