单胺类递质在大鼠脑出血心脏损伤中的作用

Effects of monoamine nervous transmitter on rats myocardial injury during cerbral hemorrhage

目的:观察脑出血条件下血浆去甲肾上腺素(NE)及血清肌酸磷酸激酶同工酶MB型(CK-MB)含量的变化,探讨脑心综合征发生、发展机制。方法:动态监测大鼠脑出血过程中血浆NE含量、血清CK-MB水平的变化,NE的测定采用高效液相-电化学法,CK-MB的测定采用酶反应速率法。结果:大鼠脑内血肿开始形成的6小时血浆NE、血清CK-MB均显著升高,并以脑出血24小时血肿高峰期时最为显著,随后逐渐下降。结论:外周NE含量升高可能参与脑心综合征的发生发展过程。

Objective: To observe the change of plasma noradrenaline (NE) and serum creatine kinase-MB (CK-MB) during cerebral hemorrhage(ICH), discuss the mechanism of Cerebral-Cardiac Syndrome (CCS). Methods: The ICH was established by infusing collagenase and heparin into rat caudate. We observed the changes of plasm NE and serum CK-MB at preoperation, 0. 5h, 6h, 12h, 48h and 72h postoperation. respectively. NE were determined by high-performance-liquid chromatography. CK-MB were determined by enzyme rate method. Results: NE activity in plasma and serum CK-MB obviously increased during the dime of intracerebral hematoma formation at 6h postoperation. and became significant during the peak time of intracerebral hemaloma formation at 24h. then gradually decreased. Conclusion: The enhanced activity of peripheral NE may play an important role on the mechanism of Cerebral-Cardiac Syndrome.