子宫内膜异位症中在位和异位内膜自然杀伤细胞的分布及数量变化

Changes of Distribution and Number of NK Cells of Ectopic and Eutopic Endometrium in Endometriosis

目的 :探讨子宫内膜异位症 (内异症 )患者异位、在位内膜组织中自然杀伤 (NK)细胞的数量变化及其在内异症发生、发展中可能的作用。方法 :采用CD57单克隆抗体 ,应用免疫组织化学法标记内异症患者的在位和异位子宫内膜组织中NK细胞 ,观察其分布特点并计数其密度 ,并与正常妇女子宫内膜对照研究。结果 :CD57阳性的NK细胞存在于内异症组和正常对照组的增殖期与分泌期子宫内膜组织中。内异症患者在位、异位内膜之间及与正常对照组子宫内膜中NK细胞的数量均无统计学差异(P>0.05) ,但内异症组的异位内膜NK细胞密度有增多的趋势。结论 :(1)内异症患者的子宫在位内膜和异位内膜的NK细胞的数量无明显变化 ;(2)内异症患者异位内膜中的NK细胞的功能可能受抑制 。

Objective:To investigate the distribution and number of nature killer(NK)cells in ectopic and eutopic endometrium in patients with endometriosis(EM),and its role on pathogenesis.Methods:NK cells marked with CD57antibody by immunohistochemical technique in endometrium were observed for the distriˉbution and density.Results:The positive NK cells with CD57were present in endometrium in proliferative and secretive stage in EM and control groups.There was no significant difference in the number of NK cells between ectopioc and eutopic endometrium in EM and control groups(P>0.05),but there was an increased trend in the density of NK cells in ectopic endometrium than that in eutopic and control endometrium.Conclusion:(1)There is no significant difference in the number of NK cells between ectopic and eutopic endometrium in patients with EM.(2)The function of NK cells in ectopic endometrium of EM may be inhibited,and ectopic endometrium can not be cleaned by NK.