幽门螺杆菌与胃癌Ha-ras和p53基因点突变相关性的研究

A correlationstudyon Helicobacter pylori infection and gastric cancer genesis relevant to the point mutation of c-Ha-ras and p53 genes

目的 探讨幽门螺杆菌 ( Hp)感染在胃癌及癌前病变中的作用机制。方法 应用分子生物学方法检测 c- Ha- ras和 p5 3基因点突变。结果 H p感染阳性患者 c- Ha- ras和 p5 3基因的突变率明显高于 Hp阴性患者 ;胃癌组 Hp感染阳性患者中 c- Ha- ras和 p5 3的突变率显著高于阴性患者 ;胃癌组织学分类之间 c- Ha- ras和p5 3的突变率无显著差异 ;癌前病变中结肠型肠化生与腺瘤样不典型增生的组织中 ,Hp阳性患者中 c- Ha- ras和p5 3突变率显著高于其他类型。结论 Hp感染与胃癌有关 ,基因突变是

Objective To explore the mechanism of Helicobacter pylori (Hp) infection in the genesis and development of gastric cancer and gastric precancerous lesion.Methods The point mutation of c-Ha-ras and p53 genes was detected by polymerase chain reaction single strand conformation polymorphism analysis (PCR-SSCP).Results The mutation rate of c-Ha-ras and p53 genes in Hp-positive cases was significantly higher than that in Hp-negative cases.For gastric cancer patients the mutation rate of c-Ha-ras and p53 genes in Hp-positive cases was significantly higher than that of Hp-negative cases.Cancer histological classification showed there was no significant difference between the mutation rates of c-Ha-ras and p53.But in gastric precancerous lesions of colon-type metaplasia and adenomatous hyperplasia the mutation rate of c-Ha-ras and p53 genes of Hp-positive patients was significantly higher than other types.Conclusion Hp infection has relation to gastric cancer,gene mutation is an important factor in Hp′s carcinogenesis.