胰腺癌p57^(kip2)和p27^(kip1)蛋白的表达及与临床病理关系研究

Expression of p57^(kip2) and p27^(kip1) proteins and its relationship with clinicopathology in human pancreatic cancer

目的:探讨p57kip2和p27kip1蛋白在胰腺癌发生发展中的作用及与临床病理特征的关系.方法:采用免疫组化技术SP法,检测p57kip2和p27kip1蛋白在32例胰腺癌组织和癌旁胰腺组织中的表达情况.结果:p57kip2蛋白阳性表达率在胰腺癌组织中为46.9%,显著低于癌旁胰腺组织(75.0%)(χ2=5.317,P<0.05),并与胰腺癌组织分化程度有关(χ2=4.979,P<0.05),而与淋巴结转移无关(χ2=3.698,P>0.05).p27kip1蛋白阳性表达率在胰腺癌组织中为56.3%,显著低于癌旁胰腺组织(84.4%)(χ2=6.063,P<0.05),并与胰腺癌组织分化程度和淋巴结转移均有关(χ2=5.776;χ2=4.097,P<0.05).p27kip1阳性胰腺癌组p57kip2蛋白表达阳性率50.0%高于p27kip1阴性胰腺癌组p57kip2蛋白表达阳性率42.9%,但二者无显著相关(r=0.19657,P>0.05).结论:p57kip2和p27kip1蛋白的低表达可能与胰腺癌发生发展密切相关;p57kip2和p27kip1蛋白表达均降低有助于胰腺癌的发生及细胞分化程度及预后的判定.

AIM: To investigate the effects of p57kip2 and p27kip1 proteins on the development and progression of pancreatic cancer. METHODS:Expression of p57kip2 and p27kip1 proteins in tumor and adjacent tissues of 32 patients with pancreatic cancer were detected by SP immunohistochemical technique. RESULTS:p57kip2 protein positive rate in tumor tissues of pancreatic cancer was 46.9 % , which was lower than that in adjacent pancreatic tissues (75.0 %)(χ2=5.317,P <0.05), p57kip2 protein expression correlated remarkably with tumor cell differentiation (χ2=4.979,P <0.05), but did not correlate with lymph node metastasis (χ2=3.698, P <0.05); p27kip1 protein positive rate in the tumor tissues was 56.3 %, which was lower than that in adjacent pancreatic tissues (84.4 %) (χ2=6.063,P <0.05). p27kip1 expression was correlated remarkably with tumor cell differentiation and lymph node metastasis (χ2=5.776;χ2=4.097,P <0.05). p57kip2 protein positive rate (50.0 %) in p27kip1 protein positive group was higher than that (42.9 %) in p27kip1 protein negative group, and there was no significant correlation between the two groups (r =0.19657, P >0.05). CONCLUSION:p57kip2 and p27kip1 proteins may play an im- portant role in carcinogenesis and progression of human pancreatic cancer. Decreased expression of p57kip2 and p27kip1 proteins is subject to the development of pancreatic cancerand determination of cell differentiation degree, and helpful to evaluate prognosis of the diseases.