人釉原蛋白重组质粒Psec Taq2A-AMG在人牙龈成纤维细胞表达的研究

Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in Human Gingival Fibroblast.

目的 研究釉原蛋白重组质粒PsecTaq2A -AMG在人牙龈成纤维细胞 (GFB)中的表达 ,为牙周再生的基因治疗奠定基础。方法 采用酶解 -组织块法体外培养得到人牙龈成纤维细胞 ,用釉原蛋白重组质粒PsecTaq2A-AMG瞬时转染牙龈成纤维细胞 ,分别在第 4 8小时、第 5天、第 7天、第 14天收集细胞及细胞液 ,经ELISA连续测定釉原蛋白的表达。结果 转染后 4 8小时即检测到表达产物 ,表达量在第 5天和第 7天达到高峰 ,持续到第 14天仍可检测到釉原蛋白的表达。结论 实验结果为利用牙龈成纤维细胞为靶细胞 。

Objective To investigate the amelogenin expression of recombinant Plasmids PsecTaq2A-AMG in human gingival fibroblast cell line. Methods Primary human fibroblasts were cultured and transfected with PsecTaq2A-AMG. The cells and cultured supernant were colllected for the detection of expression of amelogenin on day 0,2,5,7,14 by ELISA test. Results The AMG protein concentration was detected until 14 day, and peaking on the 5th and 7th day. Conclusions The results thus provided bases for the gene transfer of amelogenin into human gingival fibroblast, which may give insight for the gene therapy for periodontal regeneration.