山东四种菟丝子的RAPD分析

RAPD Analysis of Four Species of Cuscuta in Shandong Province

目的:考查山东不同种及不同寄主上的菟丝子基因组DNA是否有差异。方法:用50种引物进行RAPD分析。结果:8种引物能有效区别4种菟丝子样品;线茎亚属的菟丝子和南方菟丝子有3条相同的扩增带,分子量分别为1.3kb、1.45kb及1.53kb;单柱亚属的金灯藤和啤酒花菟丝子在1.48kb显示清晰的DNA谱带。同一亚属的菟丝子RAPD结果较接近,具有较近的亲缘关系。寄生于不同植物上的同种菟丝子显示出DNA扩增片段的多态性,表明寄主植物对菟丝子的基因组有一定影响。结论:RAPD分析可以作为性状相近的几种菟丝子,以及不同寄主上的同种菟丝子的鉴定依据。

Objective:To explore the genome difference of four species of Cuscuta in different hosts. Method:RAPD was used by 50 primers. Result: Four species of genus Cuscuta can be identified by 8 primers.Both Cuscuta chinensis and C. australis from Subg. Grommica had 3 bands whose molecular weights were 1.3kb, 1.45kb and 1.53kb respectively. C.japonica and C. lupuliformis from Subg. Monogyna had a 1.48kb specific band. Cuscuta of same subgenus had similar RAPD result and close genetic relationship. Same species of Cuscuta in different hosts showed DNA polymorphism. It indicated that hosts can affect genome of Cuscuia to some extent. Conclusion; RAPD can be used to identify the species of Cuscuta or same Cuscuta in different hosts.