大豆异黄酮对去卵巢大鼠主动脉晚期糖化终末产物(AGEs)及脂质过氧化的影响

Effects of Isoflavone (ISO) on Advanced Glycosylation End Products (AGEs) in Aortae and Serum Levels of Lipid Peroxide in Ovariectomized Rats

目的:探讨大豆异黄酮对去卵巢(OVX)大鼠晚期糖化终未产物(AGEs)和血清脂质过氧化物的影响。方法:将6月龄雌性SD大鼠随机分为假手术组(Sham)、去卵巢组(OVX)和去卵巢+大豆异黄酮组(OVX+ISO)。去除双侧卵巢2周后治疗13周。禁食24h,取血,处死动物,取主动脉,分别测定主动脉AGEs含量、血脂和血清过氧化物含量。结果:与假手术组相比,去卵巢组主动脉AGEs,血清甘油三酯(TG)、氧化低密度脂蛋白(OX-LDL)和丙二醛(MDA)含量均明显升高,高密度脂蛋白-胆固醇(HDL-C)、载脂蛋白AI(apo-AI)和超氧化物歧化酶(SOD)活性均显著降低。大豆异黄酮明显调节这些改变。结论:大豆异黄酮通过降低去卵巢大鼠主动脉AGEs含量,血清OX-LDL和MDA水平,提高SOD活性,调节血脂,预防和减少动脉粥样硬化的发生。

Objective:To investigate effects of isoflavone(ISO) on advanced glycosylation end products (AGEs) and lipid peroxide of o-variectomized rats.Methods:Rats were randomly divided into sham group (Sham),ovariectomized group (OVX) and OVX+ ISO group. Rats were ovariectomized, And two weeks later, OVX + ISO group were treated with ISO.Levels of aortic AGEs, lipids and serum lipid peroxide were detected after 13 weeks of treatment.Results:The aortic AGEs,serum triglycerides (TG) ,oxidated-LDL (OX-LDL) and malondialdehyde (MDA) of the OVX group were higher ( P < 0.01, P < 0.05, P < 0.05 and P < 0.01 , respectively) and the levels of high density lipoprotein-cholesterol (HDL-C), apolipoproptein A I (apo-AI) and activity of superoxide dismutase (SOD) were lower (P< 0.01,all) than that of the sham group.ISO not only reduced the concentration of aortic AGEs, serum OX-LDL,TC and MDA (P< 0.01 ,P< 0.05,P< 0.05 and P< 0.05 respectively) , but also increased concentration of serum HDL-C,apo-AI and SOD activity (P< 0.01, all) . Conclusion;ISO could decrease aortic AGEs,serum MDA and OX-LDL,enhance SOD activity and adjust serum lipids,therefore it maybe helpful to cardiovascular system.