川芎嗪对脐带血细胞内柔红霉素浓度影响的初步研究

Initial Study of the Effects of Tetramethylpyrazine on the Level of Daunomycin in Umbilical Cord Blood Monocytes

目的 观察川芎嗪或 和异博定与柔红霉素伍用后脐带血细胞内抗癌药物浓度 ,了解在用抗癌药物处理时川芎嗪或 和异博定的干预后抗癌症药物对正常细胞的保护或损伤作用。方法 利用柔红霉素自发荧光特点 ,以流式细胞仪测定川芎嗪或 和异博定与柔红霉素伍用体外孵育后 6例脐带血细胞内柔红霉素浓度。结果 单独使用川芎嗪能够显著降低脐带血细胞内柔红霉素浓度 ;而单独用异博定处理或异博定与川芎嗪合用时 ,对脐带血细胞内柔红霉素浓度均无显著影响。结论 川芎嗪通过降低细胞内抗癌药物浓度而发挥对正常细胞的保护作用 。

Objective To investigate the influence of tetramethylpyrazine (TMP) and/or verapamil (VP) on the level of daunomycin (DNM) in the umbilical cord blood monocytes. Method According to the fluorescent property of DNM, flow cytometry was used to detect the level of DNM in the monocytes of 6 cases' DNM containing umbilical cord blood after being incubated with TMP and/or VP in vitro. Results TMP alone could significantly decrease the level of DNM in the monocytes of umbilical cord blood; neither VP alone nor VP and TMP in combination could significantly influence the level of DNM in the monocytes of umbilical cord blood. Conclusion TMP may exert a protective effect on the normal cells by decreasing the intracellular level of anticancer drugs, of which the mechanism is waiting for being studied.