甲基苯丙胺的神经毒性及达体朗的保护作用

TOXICITY OF METHAMPHETAMINE AND THE PROTECTIVE EFFECT OF TATINOL

目的 :探讨甲基苯丙胺的神经毒性及达体朗的保护机制。方法 :设立对照组 (A)和实验组 (B、C、D、E) ,B组腹腔注射 (ip)甲基苯丙胺 ;C、D、E组分别在给予甲基苯丙胺前 7d、4d和同时给予达体朗 ;A组注射等体积生理盐水。实验后采用HE染色、银染色 ,光镜观察 ;TUNEL法检测凋亡。结果 :甲基苯丙胺可损害神经元的轴突、树突 ,并诱导凋亡。达体朗 7d组和 4d组的银染结果和TUNEL阳性细胞数与单纯给予甲基苯丙胺组比较差异有显著性 (P <0 .0 5 )。结论 :甲基苯丙胺通过诱导凋亡 ,引起神经毒性 ;

Objective : To investigate the neurotoxicity of methamphetamine and the neuro-protective effect of tatinol. Methods : An acute toxic dosing model in rat was established by intraperitoneal injection of methamphetamine, and was treated with tatinol. HE and silver staining, observed with light microscope and apoptosis were detected by TUNEL. Results : Methamphetamine damaged the brain and induced apoptosis; tatinol blocked apoptosis. Conclusion : Methamphetamine can produce neurotoxicity via apoptosis and tatinol is neuroprotection.