肺癌转移抑制基因和多药耐药蛋白的表达及意义

Expression and Implication of Multidrug Resistance-associated Protein Gene and Metastasis Suppressor KAI_1 Gene in Lung Cancer

目的 :研究肺癌转移抑制基因 (KAI1)和多药耐药蛋白 (MRP)的表达水平及内在联系。方法 :应用逆转录聚合酶链反应 (RT PCR)分别检测肺癌组织中MRP和KAI1。结果 :在肺癌组织中MRP阳性表达率为 4 5 8% (2 2 4 8) ,KAI1阳性表达率为 2 2 9% (11 4 8)。在Ⅰ、Ⅱ期患者中 ,KAI1基因为高表达 ;在Ⅲ期患者中 ,MRP基因为高表达。结论 :早期肺癌患者KAI1基因呈高表达 ,而在晚期患者MRP基因高表达 ,两者之间可能通过p5

Objective To study the expression of the multidrug resistance-associated protein (MRP) gene and metastasis suppressor KAI 1 gene,and the relationship between them.Methods MRP and KAI 1 were examined by RT-PCR.Results The positive expression rate of MRP in lung cancerous tissues was 45.8%(22/48),while the KAI 1 was 22.9%(11/48).There existed much higher expression of KAI 1 gene in Ⅰ,Ⅱ stages patients,meantime,the expression of MRP gene was higher in Ⅲ stage patients.Conclusions In lung cancerous patients of early stages,it shows that the expression of KAI 1 gene was higher and the same result of MRP gene was in later stages of lung cancerous patients.The p53 gene may mediate the expression of the two genes.