p53和血管内皮生长因子表达与胃癌血管生成的关系

Relationship of p53 and VEGF Expression with Tumor Angiogenesis in Gastric Carcinoma

目的 :探讨p5 3和血管内皮生长因子表达与胃癌血管生成的关系。方法 :应用免疫组织化学方法检测 76例胃癌组织的p5 3蛋白、血管内皮生长因子 (VEGF)与微血管密度 (MVD)表达 ,分析p5 3蛋白、VEGF及MVD表达与临床病理指标之间的关系。结果 :胃癌组织p5 3蛋白与VEGF阳性表达率分别为 4 2 1% (32 76)和 38 2 % (2 9 76) ,MVD平均计数为 36 6± 17 2 ( x±s) ;p5 3、VEGF、MVD表达与肿瘤浸润深度 (P <0 0 5 ,P <0 .0 1,P <0 .0 5 )、淋巴结转移 (P <0 0 1,P <0 0 5 ,P <0 0 1)和远处转移 (P <0 0 1,P <0 0 1,P <0 0 1)密切相关 ;p5 3蛋白与VEGF表达密切相关 (χ2 =35 3916,P <0 0 1,P <0 0 1)。结论 :VEGF与MVD表达是反映胃癌生物学行为的重要标志 ,p5

Objective To explore the relationship of p53 and vascular endothelial growth factor(VEGF) with tumor angiogenesis in gastric carcinoma.Methods Expression of p53,VEGF and MVD (microvessel density) were examined immunohistochemically in 76 gastric carcinoma specimens,correlation among the expression of p53,VEGF and MVD and various clinicopathologic factors were also analysed.Results The expression rate of p53 and VEGF in gastric carcinoma were 42.1% and 38.2% respectively,the mean value of MVD was 36.6±17.2(±s).Statistics analysis revealed that overexpression of p53,VEGF and MVD were correlated positively with the pathologic factors such as invasive depth,lymph node involvement and metastasis to liver.Furthermore,the over expression of p53 was correlated positively with VEGF.Conclusions p53 may contribute to tumor angiogenesis by means of up-regulation of VEGF.The overexpression of p53,VEGF and MVD in gastric carcinoma were correlated positively with severity of invasive depth,lymph node involvement and metastasis to distal organs,thus may serve as the poor indicators of prognosis in gastric carcinoma.