全反式维甲酸诱导前列腺癌DU-145细胞凋亡的研究

Study on the Apoptosis of Prostatic Carcinoma Cell Line DU-145 Cells Induced by All Trans Retinoic Acid

目的 :应用全反式维甲酸作用于前列腺癌细胞系DU 145细胞 ,观察维甲酸对前列腺癌细胞生长的影响。方法 :应用全反式维甲酸 (10 -5mol L)分别作用于前列腺癌DU 145细胞 36h及 72h ,分别应用光学显微镜、电子显微镜观察细胞形态及超微结构的改变 ,并用流式细胞仪检测细胞周期的改变。结果 :全反式维甲酸作用前列腺癌DU 145细胞 36h后 ,细胞生长开始减缓 ,肿瘤细胞超微结构出现细胞核固缩、染色质凝集于核膜周边等细胞凋亡早期改变。作用 72h后 ,流式细胞仪检测肿瘤细胞出现典型的亚二倍体“凋亡小峰” ,部分肿瘤细胞出现凋亡。结论 :全反式维甲酸可以诱导前列腺癌细胞产生凋亡 。

objective To observe the change of tumor cell's growth by treating prostatic carcinoma cell line DU-145 using all trans retinoic acid(ATRA).Methods DU-145 cells were treated with ATRA(10 -5 mol/L)for 36 hours and 72 hours,and changes of the cell's shape and ultrastructure were observed by optical and electron microscope.The effect of retinoic acid on the cycle of tumor cells were analyzed by flow cytometry.Results After 36 hours treated with ATRA,prostatic carcinoma cells DU-145 grew slowly.After 36 hours treated with ATRA,the cell's ultrastructure appeared some early apoptotic changes,including cell shrinkage,nuclear condensation.After 72 hours treated with ATRA,some apoptotic cells were detected,and a typical subdiploid peak were observed by flow cytometry.Conclusions Retinoic acid can induce prostatic carcinoma DU-145 cells to apoptosis.The apoptotic process has a time-depending character.