摘要
目的探索一种利用随意引物聚合酶链反应(AP-PCR)来鉴定不同血链球菌的方法。方法利用25碱基的引物5'AAGAGAGGAGCTAGCTCTTCTTGGA3',对血链球菌染色体DNA进行AP-PCR检测。结果不同菌种的血链球菌DNA经AP-PCR扩增后,产生不同的DNA片段;用不同方法提取同种血链球菌DNA可以得到相的DNA片段。结论AP-PCR可用于对血链球菌群中不同菌种进行鉴定和分类。
Objective To find out an ideal method used in identification of Streptococcus sanguis group(SSG)strains by arbitrary primed polymerase chain reaction(AP-PCR).Methods AP-PCR was used to distinguish SSG strains by designing25bp arbitrary primer5' AAG AGA GGA GCT AGC TCT TCT TGG A3'.Results There were great differences in the main band of DNA polymorphism among SSG species.The similar band can be obtained from the different DNA extractions in the same species.
出处
《上海口腔医学》
CAS
CSCD
2003年第1期21-23,共3页
Shanghai Journal of Stomatology
关键词
随意引物聚合酶链反应
血链球菌群
分类
鉴定
AP—PCR
Conclusion AP-PCR may be useful in the identification and classification of SSG species.AP-PCR
SSG
Identification
Classification
