人DDX36和小鼠Ddx36基因在成年睾丸组织中的表达研究(英文)

Expression Research for Human DDX36 and Mouse Ddx36 Gene in the Adult Testis

果蝇是结构基因组学和功能基因组学研究的最为理想的一种模式生物 ,采用同源克隆的策略 ,应用生物信息学分析和实验技术相结合的方法分别从人和小鼠中克隆了同源于果蝇MLE蛋白的新基因DDX36和Ddx36。为进一步研究DDX36和Ddx36基因与精子发生的关系 ,再应用Northernblotting、RT PCR和组织原位杂交技术探讨了DDX36和Ddx36基因的表达情况 ,结果发现人DDX36和小鼠Ddx36基因在成年睾丸组织中高表达。初步证明DDX36和Ddx36基因在精子发生中亦可能发挥重要作用。

Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for structural and functional genomic research.With the strategy of homologue molecular cloning using the sequence of the maleless gene (mle) of Drosophila,the novel homologous human and mouse genes with longer DNA/RNA helicase box (DEAD/DEAH box),named DDX36 and Ddx36 genes,respectively,were cloned as new members of the DEAD/H box superfamily.In order to further investigate the relationship between those two genes of DDX36 and Ddx36 and the role of spermatogenesis,the expression analysis of them have been performed by the techniques of Northern blotting,RT-PCR and tissue in situ hybridization.The result indicated that the DDX36 and Ddx36 gene has highly expressed in the adult testis.It was primarily suggested that DDX36 and Ddx36 gene may be related with spermatogenesis.