肾上腺嗜铬细胞瘤中c-myc、bcl-2 mRNA表达及意义的研究

Expression and Significance of c-myc、bcl-2 mRNA in Human adrenal pheochromocytomas

目的 探讨癌基因c -myc、凋亡抑制基因bcl-2mRNA在肾上腺良性及恶性嗜铬细胞瘤中的表达及其意义。方法 应用核酸分子原位杂交方法检测 2 9例良性肾上腺嗜铬细胞瘤及 7例恶性肾上腺嗜铬细胞瘤中c -myc、bcl-2基因表达情况。结果 原位杂交结果显示 :c -mycmRNA在良、恶性肾上腺嗜铬细胞瘤中表达率分别为2 7.5 % (8 2 9) ,71.4% (5 7) (P <0 .0 5 ) ;bcl-2mRNA在良性肾上腺嗜铬细胞瘤中表达率分别为 48.2 % (14 2 9,71.4%(5 7) ,两者比较存在显著性差异 (P <0 .0 5 )。结论 1、c -myc在肾上腺嗜铬细胞瘤的发生过程中c -myc基因不起主要作用 ,可能对该肿瘤的恶性变起重要作用 ,c -myc基因产物的检测对鉴别良、恶性肾上腺嗜铬细胞瘤有一定意义。 2、bcl-2基因的凋亡抑制作用对肾上腺嗜铬细胞瘤的发生发展有重要意义 ,但在良、恶性肾上腺嗜铬细胞瘤的鉴别上无临床意义。

Objective:The expression of c-myc?bcl-2 in human adernal pheochromocytmas were studied at mRNA levels.Methods:29 cases of benign pheochromocytomas and 7 cases of malignant pheochromocytomas were studied with in situ hybridization methods.Results:c-myc mRNA positive rate in 29 cases of benign and 7 cases of malignant adrenal pheochromocytomas were 27.5%(8/29) and 71.4%(5/7)(P<0.05).bcl-2 mRNA positive rate in 29 cases of benign and 7 cases of malignant adrenal pheochromocytomas were 48.2%(14/29) and 71.4%(5/7)(P<0.05).Conclusion:1. c-myc is closely related with the occurrence and development of malignant adrenal pheochromocytomas;The detection of c-myc in pheochromocytomas might be useful in differentiation of malignant from benign pheochromocytomas.2? bcl-2 overexpression is closely related to pathogenesis of adrenal pheochromocytomas.but the detection of bcl-2 in pheochromocytmas might not be useful in differentiation of malignant from benign pheochromocytmas.