期刊文献+

抗人血管内皮生长因子单克隆抗体抑制肉瘤生长的实验研究

Inhibition of Experimental Mouse Fibrosarcoma Growth by a Monoclonal Antibody to Human Vascular Endothelial Growth Factor
下载PDF
导出
摘要 目的观察抗人VEGF单抗E11对小鼠肉瘤S180生长的影响。方法BALB/c小鼠45只,接种小鼠肉瘤细胞株S180,随机均分为5组。其中3组于接种后1~4、7~11天,分别通过腹腔或瘤周皮下注射抗人VEGF单抗E11100μg、200μg。另以生理盐水及5-Fu注射作阴性及阳性对照组。接种后第14天处死小鼠并称取瘤重,计算抑瘤率,并做组织病理学检查。结果抗人VEGF单抗皮下及腹腔给药均可抑制S180肉瘤的生长,且抗人VEGF单抗200μg/d组抑瘤率均高于5-Fu组;皮下给药组抑瘤率最高,达67.2%。结论不同剂量、不同给药途径的抗人VEGF单抗对小鼠肉瘤S180的生长均有明显抑制作用,说明抗人VEGF单抗可通过阻断VEGF的作用,抑制肿瘤血管生成,从而达到抑制肿瘤生长的目的。提示抗人VEGF单抗具有可能的临床应用前景。 Objective The purpose of this study was to apply the anti-VEGF MAb(E11),which was generated against a synthetic peptide from the NH 2 of the human VEGF(residues1-26),to against VEGF in the mice fibrosarcoma S180and observe the inhibition effect of tumor growth.Methods The mice fibrosarcoma cell lines S180was injected s.c.into BALB/c mice,and then these mice were randomized into three groups.The anti-human VEGF MAb was administered i.p.or s.c.with100μg or200μg each injection on days1-4,7-11.Normal saline or5-Fu was administrated i.p.separately as control groups.Mice were sacrificed on14th day.The tumor weight was determined and the inhibition rate was calculated.Results The growth of S180fibrosacoma in MAb-treated group was dramatically inhibited,especially in MAb adminis-trated s.c.group.The tumor growth inhibition rate in mice treated s.c.with MAb reached67.2%.Conclusion The anti-human VEGF MAb had a significant inhibitory effect on growth of mouse fibrosarcoma by blocking the action of VEGF,without marked side effects.The results indicated that VEGF played an important role as a tumor angiogenesis factor,and the anti-human VEGF MAb had a potential application for clinical treatment of malignant tumors.
出处 《中国口腔颌面外科杂志》 CAS 2003年第1期35-39,共5页 China Journal of Oral and Maxillofacial Surgery
基金 国家自然科学基金(39970798)
关键词 血管内皮生长因子 血管生成 单克隆抗体 肿瘤生长 VEGF 组织病理学 Vascular Endothelial Growth Factor(VEGF) Angiogenesis Monoclonal Antibody Tumor Growth Mouse
  • 引文网络
  • 相关文献

参考文献10

  • 1[1]Zachary I. Vascular endothelial growth factor [J]. Int J BiochemCell Biol, 1998, 30(11): 1169-1174.
  • 2[2]Ferrara N, Davis-Smyth T. The biology of vascular endothelial growth factor [J]. Endocr Rev, 1997, 18(1): 4-25.
  • 3[3]Harris AL. Anti-angiogenesis therapy and strategies for integrating it with adjuvant therapy [J]. Recent Results Cancer Res, 1998,152: 341-352.
  • 4[5]Kim KJ, Li B, Winer J, et al. Inhibition of vascular endothelial growth factor-indue ed angiogenesis suppresses tumor growth in vivo [J]. Nature, 1993, 29(362): 841-844.
  • 5[6]Asano M, Yukita A, Matsumoto T, et al. Inhibition of tumor growth and metastasis by a immunoneutralizing monoclonal antibody to human vascular endothelial growth factor/vascular permeability fac tor121 [J]. Cancer Res, 1995, 55(22): 5296-5301.
  • 6[7]Kondo S, Asano M, Suzuki H, et al. Significance of vascular endothelial growth factor/vascular permeability factor for solid tumor growth, and its inhibition by the antibody [J]. Bio Bioph Rea Commu, 1993, 194(3): 1234-1241.
  • 7[8]Shinma DT, Kuroki M, Deutsch V, et al. The mouse gene for vascular endothelial growth factor. Genomic structure, definition of the transcriptional unit, and characterization of transcriptional and post-transcriptional regulatory sequence [J]. J Bio Chem, 1994,271(7): 3877-3883.
  • 8[9]Tischer E, Milchell R, Hartman T, et al. The human gene for vascular endothelial growth factor, multiple protein forms are encoded through alternative exon splicing [J]. J Bio Chem, 1991, 26:11947-11954.
  • 9[10]Sugihara T, Wadhwa R, Kaul SC, et al. A novel alternatively spliced form of murine vascular endothelial growth factor VEGF115 [J]. J Bio Chem, 1998, 273(5): 3033-3038.
  • 10王贵齐,王耐勤,刘彤,徐光炜,董志伟.血管内皮生长因子抗体对实验性肿瘤的抑制作用[J].中华肿瘤杂志,1997,19(3):163-166. 被引量:18

二级参考文献2

  • 1董志伟,中国肿瘤生物治疗杂志,1995年,2卷,84页
  • 2张梅颖,Chin J Cancer Res,1990年,2卷,1页

共引文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部