Fas转导大肠癌细胞诱导其凋亡的实验研究

The Study of Apoptosis in Colorectal Cancer Cells Induced by Fas Gene Transduction

目的:观察Fas转导大肠癌细胞株诱导大肠癌细胞凋亡效应,可望为进一步研究大肠癌的Fas基因治疗提供实验依据。方法:以流式细胞术(FCM)及琼脂糖凝胶电泳法检测Fas转导大肠癌细胞凋亡效应。实验分四组;a.LoVo细胞组b.LoVo细胞+Fas抗体组c.LoVo转导细胞组d LoVo转导细胞+Fas抗体组。结果:Fas转导大肠癌细胞株+Fas抗体组(d)细胞凋亡率明显高于a、b、c组。结论:Fas表达细胞株在Fas抗体作用下能够有效启动Fas介导的细胞凋亡途径。

Objective: To observe the apoptosis in colorectal cancer cells induced by Fas gene transduction, hoping to provide evidiences for Fas gene therapy of colorectal cancer. Methods: The apoptosis of Fas - expressed colorectal cancer cells were detected by flow cytornetry and agarose gel electrophoresis.There were 4 groups,a. LoVo cells,b. LoVo cells + Fas antibody c. LoVo Fas cells d. LoVo Fas cells + Fas antibody. Results: The apoptotic rate of LoVo Fas cells + Fas antibody group(d) was much higher than that of those three groups. Conclusion: Apoptosis in Fas - expressed colorectal cancer cells can be induced effectively by Fas gene through the function of Fas antibody.