摘要
通过番茄转化过程中农杆菌侵染浓度、预培养时间、乙酰丁香酮、植物激素和植物组织浸提液对农杆菌和外植体的处理,研究了提高番茄基因转化率的方法。试验结果为:用过夜培养的农杆菌和稀释10倍的农杆菌侵染外植体以及用1倍马铃薯伤流液和1mg/L低浓度的植物生长素处理外植体和农杆菌可以明显地提高番茄的转化率;乙酰丁香酮和番茄组织浸提液对转化率无显著影响;番茄子叶不经过预培养比预培养的转化率高。
In the experiment methods to increase transformation rate of genes in tomato were tried by adjusting Agrobacterium consistence and pre-culture time and treating Agrobacterium and explants by acetosyringone, plant hormones and extractive sap from plants. The results showed that transformation rate was enhanced when the explants were soaked in Agrobacterium , which had been cultured overnight, or in its 10-fold diluent or when Agrobacterium and the explants were immersed in the extractive sap from the tomato plants and IAA (1 mg/L). Acetosyringone and extractive sap from the tomato plants had no significant effect on transformation rate. Potato cotyledons without preculture, as explants, showed a higher transformation rate than non-pre-culture cotyledons.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2003年第2期144-146,171,共4页
Journal of Southwest Agricultural University
基金
国家杰出青年基因资助项目(39825118)
天津市教委资助项目(2001)
关键词
番茄
农杆菌介导
转基因
基因转化率
gene transformation
Agrobacterium
extractive sap from plant
hormone
